Molecular mechanisms of luteolin induced growth inhibition and apoptosis of human osteosarcoma cells
IJPR-Iranian Journal of Pharmaceutical Research. 2015; 14 (2): 531-538
em Inglês
| IMEMR
| ID: emr-167959
ABSTRACT
Luteolin is a flavone in medicinal plants as well as some vegetables and spices. It is a natural anti-oxidant with less pro-oxidant potential but apparently with a better safety profile. The purpose of this study was to investigate the molecular mechanism of luteolin-mediated apoptosis of MG-63 human osteosarcoma cells. MTT assay kit was employed to evaluate the effects of luteolin on MG-63 cells proliferation. Then, we performed Annexin V-FITC/PI to analyze the apoptotic rate of the cells. Furthermore, the inhibitory effects of luteolin on the expressions of BCL-2, BAX, Caspase-3 and Survivin were detected by Western blotting. As expected, luteolin [0.5, 2.5, 12.5 [microg/mL] inhibited the growth of MG-63 cells by inhibiting cell proliferation and inducing cell apoptosis. Western blotting demonstrated that luteolin [0.5, 2.5, 12.5 [microg/mL] inhibited the expressions of BCL-2, Caspase-3 and Survivin, and promoted the expression of BAX in MG-63 cells with a concentration dependent way. Luteolin can inhibit osteosarcoma cell proliferation and induce apoptosis effectively in a dose dependent manner through down-regulating the expression of BCL-2, Caspase-3 and Survivin proteins levels and up-regulating the expression of BAX protein level. These findings indicated that luteolin may be used as a novel herbal medicine for the treatment of osteosarcoma
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IMEMR (Mediterrâneo Oriental)
Assunto principal:
Osteossarcoma
/
Apoptose
/
Linhagem Celular Tumoral
/
Proliferação de Células
/
Crescimento
/
Antioxidantes
Limite:
Humanos
Idioma:
Inglês
Revista:
Iran. J. Pharm. Res.
Ano de publicação:
2015
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