Biological and biochemical studies on pollen grains date palm adding to rats diet

ABSTRACT

The present study was conducted on experimental animals show the effect of different levels [14%, 20% and 30%] of pollen grains of date palm on food intake, feed efficiency, serum lipid profile, serum glucose, aspartate aminotransferase [AST] and alanine aminotransferase [ALT], serum urea and createnin. Twenty eight male albino rats sprague Dawley strain weighing [95-110g] ten weeks old. They were divided into 4 groups [7 rats each], the rats of the control group were fed on standard diet, and the other 3 groups were fed standard diet plus 14%, 20% and 30% of pollen grains respectively. Food and water were provided ad-libitam for 4 weeks, at the end of experiment blood sample was taken and organs were removed. Serum was separated and kept at -20C degree until analysis. The result showed that Weight gain was highly significant in group [2] which received 14% pollen grains when compared to the control group. Higher values of food intake and feed efficiency ratio in group [2] and [3] compared to the control group. Significant decrease of serum triglyceride and very low density lipoprotein cholesterol [VLDL-c] in all treatment groups when compared to the control group. Significant decrease of low density lipoprotein cholesterol [LDL-C] in forth group which received 30% of pollen grains and significant increase of high density lipoprotein cholesterol [HDL-C] in the same group when compared to control group. Significant decrease in serum glucose were among treatments compared to control group. Significant decrease in AST in group [4]. which received 30% of pollen grains, while there was a significant decrease in ALT in all treatment groups when compared to control group. Finally, there was a significant decrease in serum urea in both group 2 and 3 when compared to control group