study of the effects of post treatment with rosmarinus officinalis aqueous extract the first 48 hours after renal ischemia/reperfusion in rats

ABSTRACT

Acute renal failure induced by ischemia / reperfusion [I/R] causes excretory functional disorders of nephrons. The purpose of this study is to evaluate the intake of oral rosemary extract [gavage] on hemodynamic changes and tissue damages caused by I/R. This study was conducted on 40 Sprague-Dawley male rats, composed of 4 groups [n=10] including control, sham, I/R+normal saline [I/R+NS] posttreatment and I/R+rosemary [I/R+RO] post-treatment. They were fed with 10 ml/kg of 8% aqueous extract or vehicle as gavages during the first 48 hours after I/R. To induce I/R, both renal artery and vein were blocked for 30 minutes followed by a reperfusion within 48 hours. Then urine and plasma samples were collected and histological study was also conducted after removal of both kidneys. Data were analyzed by SPSS-11.5 software. The one-way analysis of variance [ANOVA] and Duncan test were used for intergroup comparison and panalysis. Comparison of renal function between two groups of [I/R+RO] and [I/R+NS], showed a significant reduction in plasma creatinine [p=0.05], the blood urea nitrogen [p=0.05], absolute excretion of sodium [p=0.001] and an increase in absolute potassium excretion [p=0.01]. Results of the histological study also indicated a significant decrease in the vascular congestion, Bowman's capsule space and oxidative stress in the group [I/R+RO] in comparison with [I/R+NS]. Treatment with rosemary may be effective in the reduction of the functional and histological effects of I/R