Evaluation of antibacterial activity of extract of rumex alveollatus leaf against staphylococcus aureus and pseudomonas aeruginosa

ABSTRACT

There is considerable interest in alternative approaches for eradication of infections causes by pathogenic bacteria especially resistant bacteria. Methanol and ethanol extracts of Rumex alveollatus leaves were evaluated for in vitro antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa strains. In this empirical-experimental study, the agar plate well diffusion method was used for antibacterial assay of different sample. Broth microdilution method was used for evaluate Minimum inhibitory concentration [MIC] and Minimal bactericidal concentration [MBC] of extracts. All extracts demonstrated significant inhibitory effect against selected bacterial strains. There were statistically significant correlations between antibacterial activity and concentration of the extracts [p<0.05]. The MIC values of the extracts ranged from 31.3 mg/ml to 62.5 mg/ml whereas the MBC values ranged from 62.5 mg/ml to 125.0 mg/ml. Result from these finding suggest that methanol and ethanol extracts of Rumex alveollatus leaves may be used as natural antibacterial for treatment of some diseases, especially local skin diseases