Amelioration of glomerulosclerosis by satureja khozestanica essential oil in alloxan-induced diabetic rats

ABSTRACT

Satureja khuzestanica, an endemic plant of Iran, has been reported to be used traditionally to treat diabetes. We examined possible protective effect of Satureja khozestanica essential oil [SKE] on glomerulosclerosis in alloxan-induced type 1 diabetic rats. In this experimental study, 30 Sprage-dawley male rats were divided into 3 groups randomly; group 1 as control, group 2 diabetic untreated, and group 3 treatments with SKE by 500 ppm in drinking water, respectively. Diabetes was induced in the second and third groups by alloxan injection subcutaneously. After 8 weeks, animals were anaesthetized; livers and kidneys were then removed immediately. Kidney paraffin sections were prepared and stained by periodic acid Schiff method. Glomerular volume and leukocyte infiltration were estimated by stereological rules and glomerular sclerosis was studied semi-quantitatively. Flow treatment of diabetic animals with SKE could significantly inhibit glomerular hypertrophy [22%] leukocyte infiltration [31%] and glomerulosclerosis [20%] in comparison with the diabetic untreated group. The findings showed that SKE alleviates loss of glomerular volume, leukocyte infiltration, and glomerulosclerosis and exerts beneficial effects on the lipid peroxidation in alloxan-induced type 1 diabetic rats