[Comparative study of protein profile in Mycobacterium tuberculosis and Mycobacterium bovis with SDS-PAGE method]

ABSTRACT

Mycobacterium bovine, as the etiology of bovine TB, involves human in some cases and with Mycobacterium tuberculosis are regarded as global health problems. The aim of this study was to extract and compare protein profile of these strains in order to achieve effective biomarkers for diagnosis and the vaccine components. First, the clinical samples were cultured on Lowenstein-Jensen [LJ] medium by the N-acetyl-L-cysteine-sodium hydroxide method and the biochemical tests and antibiotic susceptibility were used. Colonies were grown in the Middlebrook 7H9 medium and, upon harvesting the new colonies, secretory and membrane proteins were extracted by sonication and ammonium sulfate and alcohol precipitation. Concentration was determined by Bradford method and finally the comparison was made through one dimensional electrophoresis. The major discrepancy between two strains of Mycobacterium tuberculosis and Mycobacterium bovis was 45 and 60 KDa bands as well as the zone between 14 and 45 KDa bands of secretory proteins, in the banding separation membrane proteins. Discrepancy in the protein bands of sensitive strains and Mycobacterium bovis seems to be used as an effective protein marker or even a biomarker in distinguishing the sensitive and bovis strains; also similarities can be used for immunization purposes: