Evaluation of carotenoids and chlorophyll as natural resources for food in spirulina microalgae

ABSTRACT

Microalgae can produce various natural products such as pigments, enzymes, unique fatty acids and vitamins that benefit humans. The objective of the study was evaluation of carotenoids [beta-carotene, zeathanthin, lutein, lycopene and astaxanthin] and chlorophyll a in spirulina microalgae. Spirulina powder has been produced by Jordan's method in Iran. Carotenoids were extracted from Spirulina platensis by adopting a method described by Reboul; then the sample was prepared and injected into a HPLC instrument with triplicate injection. Chlorophyll`s biomass content was determined by spectrophotometer. After assaying the curves of HPLC, the amount of chlorophyll a, astaxanthin, beta carotene, lycopene, zeaxanthin and lutein in spirulina was determined as 4.3 +/- 0.14, 0.21 +/- 0.02, 7393 +/- 2.76, 741 +/- 2.32, 665[+2]/-3.69 and 424 +/- 2.83 microg/ml respectively [p<0.05]