Hepatoprotective effect of aqueous extract of stem bark of berberis lycium royale in isoniazid induced hepatotoxicity in mice

ABSTRACT

To determine the hepatoprotective effect of Aqueous extract of Stem Bark of Berberis Lycium Royale in Isoniazid [INH] induced hepatotoxicity in mice. Experimental study. The study was conducted from 7[th] April 2014 till 7[th] May 2014 at animal house of National Institute of Health, Islamabad. Fifty six male albino Balb/C mice were randomly divided into four groups i.e Group I Control group [C], Group II Drug treated group [D], Group III Low Aqueous group [LA], Group IV High Aqueous group [HA] of 14 mice each. INH [50 mg/kg] was given to induce hepatotoxicity in all groups except control group [C]. Aqueous extract of stem bark of Berberis Lycium Royle was given in low doses [150 mg/kg] to Low Aqueous group [LA] and in high doses [200 mg/kg] to High Aqueous group [HA]. Blood samples were collected at day [0,15[th] and 30[th]] for estimation of Liver function tests [LFTs]. At the end samples for liver histopathology were taken. INH produced severe hepatotoxicity as depicted by raised LFT's and severe steatosis, hepatocytic ballooning and inflammation. In animals of Low Aqueous group [LA] and High Aqueous group [HA], serum levels of biomarkers were decreased and their liver sections showed improved histological picture but the reduction in toxic effects were more pronounced in animals treated with high Aqueous group. [HA]. Aqueous extract of stem bark of Berberis Lycium Royale has more significant hepatoprotective potential in high doses as compare to low doses