Serum amyloid a gene polymorphism and its association with lipid profile in Saudi females with osteoporosis

ABSTRACT

Osteoporosis can be defined as a systemic skeletal disease characterized by low bone mass and micro architectural decline of bone tissue. Serum amyloid A [SAA] is a family of protein that increases up to 1,000-fold in blood during inflammation. In this study, we aimed to study the relationship between SAA1 gene polymorphism [rs12218] and lipid profile and osteoporosis. The study was performed on the female students of Taibah University in Al Medina, KSA during June 2014 to April 2015. According to BMD; osteoporosis group [138 students] and control group [128 students]. All groups were subjected to; BMI, BMD, calcium, phosphorus, creatinine, lipid profile and SAA. Polymerase chain reaction and Real Time were done to determine the distribution of allele and genotype frequency of SAA [rs12218] C/T polymorphism. This study shows that the TT genotype of rs12218 was more frequent in osteoporosis group than control group [P<0.001]. Also, TT genotype and T allel was found to be associated with plasma total cholesterol, TG, LDLc, HDLc, Tscore, Zscore and SAA1 level in osteoporosis group [l*k].000, P=0.05, and P=0.000, P-0.000, P-0.01, P=0.02, P=0.000 respectively]. The logistic regression analysis with and without lipid disorders in the osteoporosis group also show that the TT genotype of rs12218 still differed significantly between these two groups [P=0.001,OR=1.814, 95% Cl 0.719-4.577]. The results of this study shows a significant association between TT genotype of rs12218 and both lipid level and osteoporosis in Saudi female population