Immunization trial by leishmania treated with Mitomycin-c

ABSTRACT

Leishmania tropica is a protozoan parasite that causes cutaneous disease in the old world. The study aimed to determine cell-mediated immunity by measuring serum IFN-gamma and IL-4 levels, in BALB/C mice immunized with 107 mitomycin-c treated live leishmania promastigotes [inhl], combined with 2microg IL-12, or immunized with 2 microg IL-12 only[controls], or immunized with 107 wild type leishmania promastigotes. We found that inhl induce significantly higher levels of IFN-gamma 360.62 pg/ml than controls 4.36 pg/ml and then WT 41.72 pg/ml. beside of this, inhl induce significantly lower IL-4 levels 3.18 pg/ml than WT 69.82 pg/ml and then controls [lower than 2pg/ml]. Our study revealed that inhl induce cellular mediated immunity comparable with controls and WT