Spectrofluorimetric determination of bisoprolol fumarate and valsartan in tablets and spiked plasma

ABSTRACT

Simple, sensitive and rapid spectrofluorimetric procedure is described for the determination of two antihypertensive drugs namely, Bisoprolol fumarate [BSF] and Valsartan [VT]. The effect of solvents was investigated. The fluorescence properties of the two cited drugs showed maximum emission intensity in 0.1N H[2]SO[4] at lambda [em] 298 and 415nm for BSF and VT, respectively, when excited at lambda[ex] 227nm. The calibration graphs were rectilinear from 0.08-1.28 and 0.12-1.6 micro g/ml for BSF and VT respectively. The method, was applied to the determination of the two cited drugs in tablets either single or when co-formulated with hydrochlorothiazide [HZ] with % recoveries of 100.03 +/- 0.57 and 99.70 +/- 0.90 for BSF and VT, respectively. Furthermore, the high sensitivity of the proposed method it allowed its application in spiked human plasma with good % recoveries of 99.73 +/- 2.06 for BSF and 99.94 +/- 1.71 for VT