Differential incorporation of beta-actin as a component of RNA polymerase II into regulatory regions of stemness/differentiation genes in retinoic acid-induced differentiated human embryonic carcinoma cells

ABSTRACT

Objective: Nuclear actin is involved in transcription regulation by recruitment of histone modifiers and chromatin remodelers to the regulatory regions of active genes. In recent years, further attention has been focused on the role of actin as a nuclear protein in transcriptional processes. In the current study, the epigenetic role of nuclear actin on transcription regulation of two stemness [OCT4 and NANOG] and two differentiation] NESTIN and PAX6] marker genes was evaluated in a human embryonal carcinoma cell line [NT2] before and after differentiation induction