Methylenetetrahydrofolate reductase gene polymorphism in coronary artery disease patients

ABSTRACT

Coronary artery disease occurs with the interact ion between environmental influences and genetic factors. Genetic susceptibility may be caused by mutations and polymorphisms in a variety of genes mainly involved in blood coagulation, metabolism of lipids, homocysteine and or iron. The most common form of genetic hyperhomocysteinemia results from the production of a thermolabile variant of methylene tetrahydrofolate reductase [MTHFR] with reduced enzymatic activity. This study was performed on ninety individuals selected with normal serum glucose, kidney, liver, and thyroid function test and lipid profile. They classified into Group I 27 apparently healthy persons as control group. Group II 3 apparently healthy persons with elevated homocysteine level. Group III 27 CAD patients with normal coronary angiography. Group IV 33 CAD patients with abnormal coronary angiography. The following specific investigations were done for all the studied persons- Serum homocysteine [Hcy], serum folic acid [FA] and MTHFR genotyping by PCR-RFLP