Construction of various gamma 34.5 deleted fluorescent-expressing oncolytic herpes simplex type 1 [oHSV] for generation and isolation of HSV-based vectors

ABSTRACT

Background: Oncolytic herpes simplex virus [oHSV]-based vectors lacking gamma 34.5 gene, are considered as ideal templates to construct efficient vectors for [targeted] cancer gene therapy. Herein, we reported the construction of three single/dually-flourescence labeled and gamma 34.5-deleted, recombinant HSV-1 vectors for rapid generation and easy selection/isolation of different HSV-Based vectors