Expression and secretion of cyan fluorescent protein [CFP] in B. subtilis using the chitinase promoter from bacillus pumilus SG2

ABSTRACT

Background: Improved cyan fluorescent protein [ICFP] is a monochromic, green fluorescent protein [GFP] derivative produced by Aequorea macrodactyla in a process similar to GFP. This protein has strong absorption spectra at wavelengths 426-446 nm. ICFP can be used in cell, organelle or intracellular protein labeling, investigating the protein-protein interactions as well as assessing the promoter activities