Protective effects of ethyl pymvate on sperm quality in cyclophssgphamide treated mice

ABSTRACT

AbstrnetBackground: One of the affecting factors in disturbance process of spermatogenesisis chemotherapeutic-induced oxidative stress resulted from cyciophosphamide [CP]; treatment which leads to diminisled sperm quality via interference in spermatogenesis process. Objective: This study was conducted to investigate the effects of ethyl pyruvat [EP] in reducing the CP-induced side effects on reproductive system.Materials and Methods: 24 mature male mice were randomly divided into 3 equal j groups and were undergone therapy for 35 days. Control group received normalsaline [0.1 mliday, IF]. CP group were injected CP [15 mgikglweek, IP] and CP+EPgroup received EP [40 mgilcglday, IP] as well as CP. In the end of the treatment period, the mice were euthanized by cervical dislocation. Then, the epididymis wasincubated with C02 in a human tuba1 fluid medium [1 ml] for half an hour in order to float sperm. Then, the number, motility, viability [eosin-nigrosin staining], DNAbreakage [acridine orange staining], nucleus maturity, and sperm morphology I [aniline blue staining] were analyzed. Results: The average [15.871-1.28], motility [35.77+2.75], viability [4013.03], I nucleus maturity [36*2.79] and sperm morphology [61.751-0.85] were decreasedI significantly in CP group in comparison with control and EP groups, whereas I caused significant increase of these parameters. Also, the percentage of DNA I damage was increased significantly in CP group [41.75*3.75] in comparison with I control [2*0.71] and EP groups [22.514.13]. Conclusion: The results of this study revealed ameliorating effects of EP on sperm quality of CP treated animals