Role of interferon gamma and gamma9/82 T cells in management of pulmonary tuberculosis
EJMM-Egyptian Journal of Medical Microbiology [The]. 2012; 21 (3): 89-97
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| IMEMR
| ID: emr-194375
Biblioteca responsável:
EMRO
Background: Early diagnosis and treatment are important in prevention of tuberculosis [TB] infection. Recently, many new strategies are validated for diagnosis and monitoring of pulmonary TB cases
Objective: To evaluate the role of Quantiferon-TB Gold in Tube test [QFT-GIT] and y9/32 T cells percentage in diagnosis of pulmonary TB and as tools to monitor the efficacy of anti-tuberculosis treatment
Methods: Two groups [40 patients and 15 healthy control] were enrolled in this study from July, 2009 to February, 2011. Both groups were evaluated by tuberculin skin test [TST], QFT-GIT assay, and y9/d2 T cells byflowcytometry. QFT-GIT assay, and y 9/82 T cells by flowcytometry were done twice for the pulmonary TB patients, first at the start of treatment and second after Smonths of treatment
Results: Among the forty proved pulmonary TB cases, 31[77.5%] cases were only positive for acid-fast bacilli [AFB] smear. Meanwhile, 31[82.5%] cases were positive by TST with specificity and sensitivity of 82.5% and 40% respectively in comparison to 37[92.5%] patients showedpositivity by QFT-GIT assay "tviih specificity and sensitivity of 92.5% and 93.5% respectively. Regarding, the 27 clinically improved pulmonary TB patients, 10 cases turned negative for QFT-GIT after 3 month course of antituberculous treatment. Moreover, Quantitative QFT-G level fall significantly in active tuberculosis patients undergoing treatment [p <0.05]. Whereas, the mean percentage of y 9/62 T declined mildly from 1.31 %to 1.03 % at the start and after 3 months of treatment respectively [P >0.05].Also, it -was noted that the mean percentage ofy9/d2 T decrease with the severity of the disease, 0.36%, 1.37% and 1.57% in stage 3, 2 andl respectively
Conclusion: QFT-GIT assay is a potent immnnodiagnostic test for pulmonary TB but it may not afford enough level of feasibility regarding cure of infection. However, the pretreatment concentration of IFN-y correlates to reversion to negative QFT-GIT. In addition, y9/62 T cells percent which had limited value to monitor the efficacy of anti-TB treatment but these cells could be used to assess the severity of the disease?
Objective: To evaluate the role of Quantiferon-TB Gold in Tube test [QFT-GIT] and y9/32 T cells percentage in diagnosis of pulmonary TB and as tools to monitor the efficacy of anti-tuberculosis treatment
Methods: Two groups [40 patients and 15 healthy control] were enrolled in this study from July, 2009 to February, 2011. Both groups were evaluated by tuberculin skin test [TST], QFT-GIT assay, and y9/d2 T cells byflowcytometry. QFT-GIT assay, and y 9/82 T cells by flowcytometry were done twice for the pulmonary TB patients, first at the start of treatment and second after Smonths of treatment
Results: Among the forty proved pulmonary TB cases, 31[77.5%] cases were only positive for acid-fast bacilli [AFB] smear. Meanwhile, 31[82.5%] cases were positive by TST with specificity and sensitivity of 82.5% and 40% respectively in comparison to 37[92.5%] patients showedpositivity by QFT-GIT assay "tviih specificity and sensitivity of 92.5% and 93.5% respectively. Regarding, the 27 clinically improved pulmonary TB patients, 10 cases turned negative for QFT-GIT after 3 month course of antituberculous treatment. Moreover, Quantitative QFT-G level fall significantly in active tuberculosis patients undergoing treatment [p <0.05]. Whereas, the mean percentage of y 9/62 T declined mildly from 1.31 %to 1.03 % at the start and after 3 months of treatment respectively [P >0.05].Also, it -was noted that the mean percentage ofy9/d2 T decrease with the severity of the disease, 0.36%, 1.37% and 1.57% in stage 3, 2 andl respectively
Conclusion: QFT-GIT assay is a potent immnnodiagnostic test for pulmonary TB but it may not afford enough level of feasibility regarding cure of infection. However, the pretreatment concentration of IFN-y correlates to reversion to negative QFT-GIT. In addition, y9/62 T cells percent which had limited value to monitor the efficacy of anti-TB treatment but these cells could be used to assess the severity of the disease?
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Índice:
IMEMR
Tipo de estudo:
Screening_studies
Idioma:
En
Revista:
Egypt. J. Med. Microbiol.
Ano de publicação:
2012