Evaluation of immune response of bacterial toxin utilized as immune-potential adjuvant

ABSTRACT

The present work aimed to evaluate the adjuvanticity of r-CTB compared with currently used alum; laboratory animals were subcutaneously immunized using r-CTB and alum adjuvenated diphtheria vaccine candidates. Maximum antibody level was detected on the 5[th]-6[th] week post immunization with r-CTB used as 1,5,and 20 micro gm, and the Ab level post r-CTB was significantly elevated than alum adjuvanted vaccine [P<0.05]. Antibody and IFN-gamma level were r-CTB dose dependent, while IL-10 level was not. IFN-gamma level was significantly higher and long durative in case of immunization with r-CTB adjuvanted diphtheria vaccine than that detected post immunization with alum adjuvanted diphtheria one and post challenge as well. While, IL-10 level in case of adjuvation with r-CTB and Alum was almost the same concentration and need the same duration to reach the base line 0 level post immunization