Phytochemical investigations on chemical constituents of Achillea tenuifolia lam

ABSTRACT

Achillea tenuifolia Lam [Asteraceae] afforded a methanolic extract from which after fractionation in solvents with different polarities two known flavones 3', 5- dihydroxy- 4', 6, 7- trimethoxy flavone [eupatorine, compound 3], 5- hydroxy- 3', 4', 6, 7- tetramethoxyflavone [compound 4], besides stearic acid [compound 1 ], lupeol [compound 2], daucosterol [beta- sitosterol 3-O- beta- D- glucopyranoside, compound 5], 2, 4- dihydroxy methyl benzoate [compound 6] were isolated for the first time. The structure of isolated compounds was elucidated by means of different spectroscopic methods such as UV, IR, Mass and [1]H- NMR [1D and 2D] and [13]C-NMR. For further confirming of structures of isolated compounds, comparison of the spectral data of them with those reported in the litratures have been done