Studies on proteases formation by phanerochaeta chrysosporium

ABSTRACT

Glucose and sawdust media supplemented with nitrogen sources were used for the growth and proteases formation by Phanerochaete chrysosporium. Urea and ammonium sulfate were found to be suitable sources for fungal growth respectively, followed by albumin. Changes in pH values were detected during growth. Optimum protein content was reached after four days of incubation at 30 degrees in case of sawdust medium supplemented with [NH4]2SO4, while as protein content was increasing gradually by increasing the incubation period up to seven days in glucose medium supplemented with urea. Variable levels of extracellular and endocellular protease A were detected using these media, but a comparatively low level was observed in case of protease B and C. Optimum pH for endocellular protease A and B activities were found to be three and seven, respectively. A linear relationship was obtained when endocellular protease B activity was assayed at different incubation periods after one hour of incubation