Skeletal muscle CLARITY: a preliminary study of imaging the three-dimensional architecture of blood vessels and neurons

ABSTRACT

Objective: Passive CLARITY is a whole-tissue clearing protocol, based on sodium dodecyl sulfate [SDS] clearing, for imaging intact tissue containing transgenic or immunolabeled fluorescent proteins. In this study, we present an improved passive CLARITY protocol with efficient immunolabeling without the need for electrophoresis or complex instrumentation