Epigenotoxic effect of dimethyl sulfoxide on buffalo somatic cells and buffalo-bovine interspecies somatic cell nuclear transfer embryos

ABSTRACT

Objective: In the present study, we investigated the possible epigenotoxic effect of dimethyl sulfoxide [DMSO] on buffalo fibroblast cells and on reconstructed oocytes during buffalo-bovine interspecies somatic cell nuclear transfer [iSCNT] procedure and its effect on rate and quality of blastocyst which derived from these reconstructed oocytes