feasibility study to evaluate bacillus subtilis as a host for producing recombinant human parathyroid hormone

ABSTRACT

Background: Biosynthetic teriparatide [1-34] [TPD] is a N-terminally truncated version of human parathyroid hormone [hPTH]. The recombinant form of this polypeptide has been expressed in Escherichia coli [E. coli] and approved as the first anabolic treatment of osteoporosis in the EU and the USA. Feasibility of expression and secretion of a tag- fused form of TPD into Bacillus subtilis [B. subtilis] was examined due to several advantages of B. subtilis over E. coli in production of recombinant proteins with pharmacological activities