[Designing and producing of RNA interference [RNAi] in order to reduce the expression of a candidate gene involved in cancer]

ABSTRACT

Background: cancer is one of the most important factors which affect the human health .RNA interference [RNAi] based therapies have been developed and showed significant results in this regard. One of the most important problems of siRNA i system is its low stability. In order to overcome to this weakness, venous types of hairpin RNA or shRNA was introduced Over expressed colorectal cancer gene [OCC-I], which has several transcripts is considered as a hallmark of colorectal cancer. This gene has vital role in the development and progression of cancer through the cell signaling pathways, which are involved in cell proliferation. In the present study, the RNAi system was utilized in order to reduce the expression of specific transcript of OCC-1 gene