nitrosoguanidines mediated adaptive repair in pseudomonas syringae

ABSTRACT

Adaptive repair activity has been demonstrated in wild type cells of Ps. syringae. MNNG mediated [adapted] cells resisted more than the nonadapted cells. Treatment of the cells with protein synthesis inhibiting antibiotic chloramphenicol at a concentration of 100 micro gml -1 during adaptation prevented the enhanced resistance to killing effects of MNNG. However, such an effect was not demonstrated when the cells were treated with chloramphenicol during the MNNG challenge treatment of the adaptive cells. It is, therefore, evident that adaptive repair in Ps. syringae is mediated as a result of the induced synthesis of enzymes