Degradation of shrimp-shell waste and production of chitinase by bacillus amyloliquefaciens under some cultural conditions

ABSTRACT

The study of chitinase production by Bacillus amyloliquefaciens grown on shrimp-shell waste as the sole carbon source showed that chitinase production with maximum enzyme activity was reached when 2% inoculum of 24 h age seed culture was used. The inoculated medium contained [g/I] shrimpshell waste, 80; NH[4]CI, 1.2 KH[2]PO [4]; K[2]HPO[4], 1.6; MgSO[4].7H[2]O, 0.1 ZnSO[4]. 7H[2]O, 0.1; ZnSO[4] .7H[2]O. FeCI[3]. 6H[2]O, 0.01; CaCI[2]2]H[2]O, 0.02 at pH 7 and the culture shaken at 30°C for 36 hours