Some factors influencing chitinase production by bacillus amyloliquefaciens
Bulletin of the Faculty of Science-University of Alexandria. 1992; 32[A]: 240-248
em Inglês
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| ID: emr-23313
ABSTRACT
The effect of several analogues and breakdown products of chitin on the iodsetioa of the chitinase of B. amyloliquefaciens was examined. Glucose. N -acetyl-glucosamine and chitobiose inhibited the enzyme productivity. The effect of the addition of some natural extracts was also investigated and the maximum enzyme activity was produced with 2% sand extract. A simplified medium composed of 8% shrimp-shell waste and 80% sand extract yielded an enzyme of highest activity. Reaction conditions supporting best activity sum achieved using 5-7-5mg swollen chitin in 0.03 M phosphate buffer at pH 5.916.98; enzyme concentration of 11.35mg protein/reaction mixture and incubating the mixture at 45°C for 60 min
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Bacillus
/
Quitinases
Idioma:
Inglês
Revista:
Bull. Fac. Sci.-Univ. Alex.
Ano de publicação:
1992
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