specific gastric inhibitory polypeptide antiserum recognising a single molecular form

ABSTRACT

Gastric inhibitory polypeptide [GIP], a hormone of the proximal small intestine, potentiates glucose-induced insulin secretion in the presence of hyperglycaemia. It has been difficult to define the precise role of GIP due to the presence of a number of immunoreactive GIP components with differential immuno reactivity. We have raised an antiserum [S705] in sheep by multiple subcutaneous injections of natural porcine GIP after conjugating it to human albumin by the difluorodinitrobenzene [DFDNB] method. S705 recognizes only one component, immunoreactive GIP 5000, the major immunoreactive component of GIP which plays an important role in the enteroinsular axis