Sandwich ELISA technique in the detection of circulating immune complexes in schistosoma mansoni infection

ABSTRACT

Fifty sera of cases infected with S. Mansoni were tested for the detection of circulating immune complexes [CIC] by sandwitch ELISA technique. Ten more sera of healthy volunteers were used as a control. Out of 50 cases 64% were positive whereas 22% were found negative for CIC. Most of the negative cases were having low egg count in the stools. Sandwitch ELISA technique was reproducible and the percent of positivity could be raised if IgE enzyme labeled conjugate is used