Animal-passage of campylobacter strains with iron-dextran enhances the expression of an outer membrane protein
Pakistan Journal of Pharmacology. 1992; 9 (2): 29-38
em Inglês
| IMEMR
| ID: emr-26006
ABSTRACT
The outer membrane proteins [OMP] of four C. jejuni/coli isolates have been identified by SDS - PAGE using Sarkosyl extraction of crude membranes. Sarkosyl insoluble fractions of four strains consistently contained a major OMP in the range of 43 k to 48 k, in addition to some minor proteins. SDS - PAGE profile of animal - passed virulence enhanced strains, when compared with the original isogenic strains when compared with the original isogenic strains, revealed the presence of a protein band migrating at 66 k to 68 k in addition to the characteristic major OMP band, suggesting a selective enhancement of this protein during animal passage in presence of iron - dextran. This shift in OMP expression was associated with enhanced virulence of C. jejuni for BALB/c mice. Immunobloting to analyse the antigens in OMP showed antigenicity to the major OMP. The role of the 68 k OMP in the pathogenesis of C jejuni for BALB/c mice remains to be studied
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Proteínas da Membrana Bacteriana Externa
/
Ferro
/
Camundongos
Idioma:
Inglês
Revista:
Pak. J. Pharmacol.
Ano de publicação:
1992
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