Effect of percoll wash on sperm motility in artificial insemination

In this work the semen of 25 patients with initial motility 60% or less were subjected to Percoll gradient wash for sperm preparation for assisted reproductive technology. We have assessed the three motility parameters [percentage, types, longevity] before and after the wash. The mean sperm number was 50.9 +/- 16.4 million/ml. The initial sperm motility before the wash was ranging from 5% to 60% with a mean 41.12% +/- 16.44%. After percoll gradient wash sperm rnotility has been increased to 61.8 +/- 29.96% [P < 0.0025]. Percoll gradient wash eleminated sperms with motility grade O and grade 1. only sperms with motility grades 2,3 and 4 were present. We recommend the use of Percoll wash for sperm lipreparation especially in patients with asthenospermia whom will be treated by AIH