One step differential [OSD] stain for fungal elements

ABSTRACT

This stain have been invented by the author after many trials over two years to get a stain that allows clear study of microscopic structures of fungi. The stain is applied directly on fungal structures placed on the slide with no need for fixation; viz; can be used as a mount. Also, the stain has the ability to differentiate between various internal structures without a decolourization step. The viable structures and fresh cultures take the blue colour while other structures stain faint reddish to faint blue or greenish