Production of cellulases and beta-glucosidase by new isolate of Aspergillus niger F-92

ABSTRACT

The production of cellulase was investigated in shakes culture of a new isolate of Aspergillus niger F-92. Amongst different concentration of cellulose powder tested, concentration 1.5% [w/v] showed maximum production of enzyme after nine days incubation at 30C. The optimum initial pH for enzyme production was found to be 5.0. The production of cellulases was sensitive to nitrogen source in the medium, the optimum production was seen when soybean flour [600 mg N/L] was used as the only nitrogen source. KH2PO4 [0.2% w/v] was found to be optimum at concentration 0.3 M caused marked increase in cellulases and beta-glucosidase production. Under the optimum culture conditions the activities of FPase, CMCase and beta-glucosidase were 1.89, 4.2 and 7.82 IU/ml, respectively