Validation of methoxsalen national reference standard

ABSTRACT

Methoxsalen [9 methoxypsoralen] National Reference Standard was prepared from locally produced bulk after due purification. The purity of the available bulk was assessed by various analytical techniques. Purification was carried out by selective elution from a silica gel column. The purified product was compared with the current lot of U.S.P. Methoxsalen Reference Standard using UV, IR, TLC, GLC, HPLC and melting point determination. As the purified substance proved to be comparable to the U.S.P. Standard, it was recognised as National Reference Standard