Coumarins form the in-vitro culture of convolvulus arvensis L

ABSTRACT

Further investigation of the previously reported in vitro culture of Convolvulus arvensis L. revealed the presence of umbelliferone and scopolet in their identity was confirmed by co-chromatography with authentic samples and their UV data. The composition of the growth regulators, inclusion of yeast extract, sucrose content of the medium and induction of the culture greening by its illumination were found to have a prominent effect on the accumulation level of the coumarins. The greened callus was found to be unable to synthesize neither flavonoids nor alkaloids