Survival rate after acute hemorrhage in rabbits treated by xanthin oxidase inhibitor [allopurinol]

ABSTRACT

Morbidity and mortality after hypoxia of the vital organs were correlated to the production of oxygen radicle which is mediated by xanthine oxidase activity. In this study, the survival rate after allopurinol have been evaluated. 42 rabbits weighed 900 + 200 g divided into two groups. Group I, included 22 rabbits, were treated with allopurinol 80 mg/ug for seven days before induction of hemorrhage. Group II, as a control group, included 20 rabbits. All rabbits were subjected to 25% arterial blood loss through the central ear for one hour then resusciation was done by the heparinized withdrawn blood through a marginal ear vein. During the experiment, blood pressure and heart rate were monitered through the central ear artery. Also, uric acid, lactic acid, glutathione activity were estimated. Animal survival was followed for 10 days. Postmortem vital organ histochemistry and histopathology examinations were done. In group I, the survival after three days was 10 out of 22, while in group II it was out of 20. It was concluded that allopurinol had increased the survival in allopurinol pretreated rabbits which may indicate the value of allopurinol premedication for patient prepared for elective bloody surgical intervention