Evaluation of specific fasciola gigantica antigens for the diagnosis of fascioliasis in experimentally and naturally infected sheep by ELISA

ABSTRACT

The value of peak II and E/S Fasciola antigens for diagnosis of experimental and natural sheep fascioliasis were studied by ELISA. The antibody levels of experimentally infected sheep with F. gigantica which appeared at 2 weeks post-infection, gradually increased and the highest concentration of antibodies was found 9 - 13 weeks post- infection with both antigens. Also, both antigens gave 100% sensitivity for diagnosis of naturally infected sheep with fascioliasis. On the other hand, peak II antigen gave 100% specificity, while E/S antigen gave 94.29% specificity