Effect of tiaprofenic acid [surgam] on the albino rat kidney [a light and electron microscope study]

ABSTRACT

To reveal the effect of tiaprofenic acid [surgam] administration on the renal tissue, thirty healthy adult male albino rats divided into 3 groups were utilized. Control group [10 rats] received only distilled water [1 mg/kg body weight] I.M. daily for 6 weeks, treated group [10 rats] received [15 mg/kg body weight] I.M. daily for 6 weeks and examined immediately after stopping treatment and recovery group [10 rats] received the same course of tiaprofenic acid as the treated group but they were examined 3 weeks after stopping treatment. The animals were anaesthetized with ether. Kidney specimens were processed for preparation of paraffin sections which were stained with Hx and E, P.A.S. and Masson trichrome stain for histological study. Other minute specimens from the kidneys were processed for transmission electron microscope study. Areas of necrosis were detected with increased interstitial fibrosis, thickening of glomerular capillaries basement membranes and hypertrophied glomeruli. Ultrastructural changes were also found in the form of fusion of podocytes foot processes, localized thickening of glomerular capillaries basement membranes, degenerated tubular cells with affected nuclei and mitochondria. These changes did not disappear in animals examined 3 weeks after stopping of treatment. Patches of necrosis, hyaline tubular casts and interstitial hemorrhage were also found. The ultrastructural examination revealed focal fusion of podocytes foot processes and thickened glomerular capillaries basement membranes as well as distorted mitochondria and vacuolated cytoplasm of proximal convoluted renal tubules cells