Freeze-etch replication of PNH erythrocytes for transmission electron microscopy
Journal of the Faculty of Medicine-Baghdad. 1995; 37 (2): 265-274
em Inglês
| IMEMR
| ID: emr-37751
ABSTRACT
Freeze-etch replication of paroxysmal nocturnal hemoglobinurea [PNH] erythrocytes by a new freeze-fracture technique for transmission electron microscopy revealed two types of cells 1. Macrocytes with round pits. 2. Microcytes with a single elongated spiculum and well defined wart-like spherical projections about 100 nm in diameter and intramembranous particles. A ridge separating the fracture face from the etched face should be achieved by proper etching to avoid ice crystallization
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Microscopia Eletrônica
/
Eritrócitos
/
Técnica de Congelamento e Réplica
Limite:
Humanos
Idioma:
Inglês
Revista:
J. Fac. Med.-Baghdad
Ano de publicação:
1995
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