Spectrophotometric and spectrofluorometric determination of prazosin

ABSTRACT

Three simple and accurate methods have been developed for the determination of prazosin in tablets.the first method is based on the formation of anion-pair salt between the drug and bromocresol purple with subse quent absorbance measurements at 410 nm.the second depends on the reaction of prazosin with mercurochrome [mer] in aqueous alkaline medium to give an antense red color measured at 562 nm.the third method is based on the fluorescence quenching of mer in an aqueos neutral buffered medium. The decrease in mer intensity was directly proportional to prazosin concentration. The different experimental parameters were carefully studied and incorporated into the procedure. Under the described condition ranges of 2-10, 5-10 and 0.05-0.2 micro gml-l for the three methods, in order