Axenic in vitro culture of schistosoma mansoni miracidia
Journal of the Egyptian Society of Parasitology. 1996; 26 (3): 575-584
em Inglês
| IMEMR
| ID: emr-41355
ABSTRACT
Miracidia of Schistosoma mansoni have been grown axenically. The use of saline solution of 0.75% facilitated the shedding of epidermal plates which if occurred promote a rapid sporocystogenesis. Also, the use of a ratio of 1 3 [fetal calf serum and medium 199], in addition to chernin balanced salt solution [SBSS] was ideal for maintaining sporocysts up to 14 days. All the miracidia shed their epidermal plates 2 hours post cultivation. At the end of the second week post cultivation, the daughter sporocysts measured 690 u in length by 50 u in width. The usage of plastic flat sided flasks with plenty of culture medium was very important for inducing a proper growth of sporocysts
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Esquistossomose
/
Caramujos
/
Interações Hospedeiro-Parasita
Idioma:
Inglês
Revista:
J. Egypt. Soc. Parasitol.
Ano de publicação:
1996
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