Evaluation of testicular histology and micronucleus test in mice treated by bromocryptine mesylate and dothipen hydrochloride

ABSTRACT

The present study aimed to evaluate and compare the genotoxicity and morphological changes in mouse testis after short term chronic administration of either BCM or DPH. They were administered by daily oral doses [1/10 LD 50] for 4 weeks, then the treatment was stopped for another 4 weeks to study the effects of the drugs. 100 male mice were used and classified into 3 groups, a control group and 2 groups for testing the effects of BCM and DPH. The seminiferous tubules showed disorganization, hypospermtogenesis and spermatogenic arrest. Their lumina contained desquamated spermatogenic cells or necrotic tissue. The inter-tubular spaces contained dilated blood vessels and an exudate. In DPH treated group the nuclei of germ cells appeared pyknotic at the 4th week. There were marked improvement in testicular structure after cessation of BCM treatment. While the DPH-treated group needed longer period to attain its original architecture. The micronucleated PCEs were significantly increased at the 3rd and 4th weeks of BCM treatment and their numbers declined when the treatment was stopped for another 4 weeks