effect of different neurotransmitters on-some hormonal profile of immature female rats

ABSTRACT

The first objective of the present investigation was to evaluate the relative contributions of the ovarian steroid in the feed back limb of the ovarian pituitary axis. The second was to test the ability of morphine or L. dopa or both in modulating PRL, PROG, FSH and LH levels noticed in female rats previously treated with estrogen during prepubertal period. Forty immature female rats were divided into five equal groups and exposed to different treatments 1] Rats were injected i.m. with 0.2 ml olive oil and served as a control group; 2] Rats were injected with 5 mug estrogen in 0.2 ml olive oil/day; 3] Rats were injected i.m. with 5 mug estrogen in 0.2 ml olive oil/day/rat + morphine sulfate at a dose pf 10 mg/kg b. wt.; 4] Rats were injected i.m. with both 5 mug estrogen in 0.2 ml olive oil/day/rat + L. dopa at a dose of 30 mg/rat/day; 5] Rats were injected i.m. with 5 mug estrogen in 0.2 ml olive oil/day/rat + morphine sulfate at a dose of 10 mg/kg b. wt. + L. dopa at a dose of 30 mg/rat/day. Animals were inspected daily for vaginal opening and estrous manifestation. Blood samples were collected at the end of experimental period and estimated by radioimmunoassay kits for PRL, LH, FSH and PROG. The data obtained revealed that sexual precocity elicited either with estrogen or estrogen +/- L. dopa treatment of the neurotransmitter in hypothalamic sensitivity to estrogen in induction of puberty may be considered