In vivo induction of sister chromatid exchanges analysis of mice bone-marrow chromosomes, primary spermatocytes and micronucleus test: accurate cenotoxic bioassays for DNA damage induced by the drug alexoprine

ABSTRACT

Accurate genotoxic bioassays for measuring DNA damage [in vivo induction of sister chromatid exchanges, analysis of mice bone marrow chromosomes, analysis of mice primary spermatocytes, and micronucleus test] were employed and used. Analysis of sister chromatid exchange frequencies revealed that Alexoprine is capable of inducing primary DNA damage. The results showed that the tested drug has clastogenic activity upon mice bone marrow chromosomes. Analysis of mice primary spermatocytes revealed that Alexoprine is capable of affecting the germinal cells. Analysis of micronucleated polychromatic erythrocytes presented an evidence that Alexoprine is a clastogenic agent. The tested drug Alexoprine is capable of inducing primary DNA damage, as SCEs revealed it has clastogenic activity upon somatic and germinal cells as well