Viral incrimination in some oral precancerous and cancerous lesions

ABSTRACT

One hundred and seventeen cases were immunologically analyzed using H and E stain, PAP technique, as well as in situ hybridization. In situ hybridization was used to detect HPV [Human Papilloma Virus] DNA on 11 cases of epithelial dysplasia, 9 cases of carcinoma in situ, 50 cases of squamous cell carcinoma and a rare case of carcino-sarcoma. Immunopositive signals were noticed in about 25% of all these cases, in addition the carcino-sarcoma showed positivity for type 16 and 18 probes. Immunocytochemical studies were done for EBV [Epstein Barr-Virus], HSV [Herpes Simplex Virus] and HPV antibodies on the same previous cases, in addition to variants of squamous cell carcinoma [17 cases], oral malignant melanoma [9 cases], hemangioendothelioma [HE] [6 cases], embryonal type of rhabdomyosarcoma [9 cases], as well as 5 cases of malignant odontogenic tumours, excluding the carcinoma case. Positive immunocreation was observed in the 11 cases of epithelial dysplasia [2 cases positive for EBV, 4 cases positive for HSV, and 5 cases positive for HPV]. Out of the 9 cases diagnosed as carcinoma in situ, one case was positive for EBV, 3 cases positive for HSV, and 5 cases positive for HPV. The squamous cell carcinoma with its variants revealed 40% positivity for HPV, 20% for HSV and 15% for EBV. Five out of the nine cases of malignant melanoma showed positive reaction for EBV only. Five out of 6 cases of HE were positive for EBV. Five cases out of the 9 embryonal rhabdomyosarcoma showed expression for HPV, and the remaining 4 cases showed positivity for EBV. All the malignant odontogenic tumour cases revealed positive reaction for both HPV and EBV