High pressure liquid chromotographic analysis of DNA from different snail species, target and non-target to schistosoma infection

ABSTRACT

DNA of target and non-target snail species was analysed for the ratios of the different nitrogenous bases [adenine, guanine, cytosine and thymine] by HPLC. The effect of two newly synthesized antimetabolites namely [methyl [5-aminopyrazol-5-yl] aminouracil]-4-carboxylate [I] and methyl [7-methyl-2-[5-uracilylamino] pyrazolo [1, 5-a] pyrimidin-5 one]-3-carboxylate [II]], and also that of bromouracil and aminouracil on the chemical nature of DNA of Biomphalaria alexandrina snails, as the most prevalent target species, was studied through treatment of snails with bait formulations containing attractant materials and different concentrations of the selected chemical compounds. The results pointed out that incorporation of these chemicals inside attractant food pellets [AFP] did not cause any repellent effect to snails. The results showed also, that the percentages of DNA bases varied among the different snail species and that the different treatments induced some alterations in the ratios of these bases