SDS gel analysis of matrix of adherent cell lines with particular reference to mesangial cells

ABSTRACT

Cultured mesangial cells produce a variety of extra cellular matrix proteins and secretion are increased by cytokines. To identify the molecular weight of proteins in the matrix of stimulated and non-stimulated mesangial, BeWo, Hep G2 and BHK cells was studied using SDS and praline gel analysis. The results showed a common band of 65-70 kd in the matrix of both stimulated and non-stimulated mesangial cells and other cell lines, while a unique 150 kd band was obtained in the matrix of stimulated mesangial and BeWo only. These data indicated the effect that cytokines have on the expression of different proteins