Journal of the Egyptian Society of Parasitology. 1997; 27 (1): 153-6
em Inglês
| IMEMR
| ID: emr-44942
ABSTRACT
The objective of this work was to test the immunogenic effect of 2 purified non-infected B. alexandrina hepatopancreas through histopathological changes in liver of Swiss albino mice [15 - 20 g]. Gel filtration chromatography was used to fractionate the crude antigen into 5 fractions, followed by re-fractionation and determination of their molecular weights by sodium dodecyl sulfate polyacrylamide gel electrophoresis [SDS-PAGE]. Four dilutions of Fiv [20000 - 29000 daltons] and Fv [20000 - 240000 daltons] were injected in 2 groups of mice [33 each] at weekly intervals and another control group was injected with phosphate buffer saline [PBS] in the same manner. Sacrification was done at 7 weeks from infection with 100 S. mansoni cercariae through immersion method. The results revealed that there were marked histopathological changes in liver of the control group in comparison with the 2 vaccinated groups, which appeared more or less normal with slight inflammatory infiltrate
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Schistosoma mansoni
/
Caramujos
/
Cromatografia em Gel
/
Vacinação
/
Fígado
/
Camundongos
/
Antígenos
Limite:
Animais
Idioma:
Inglês
Revista:
J. Egypt. Soc. Parasitol.
Ano de publicação:
1997
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