Micro-determination of reserpine in pharmaceutical formulation

ABSTRACT

A rapid gas liquid Chromatographic [GLC] method for the determination of reserpine is proposed. The method depends on the trans-esterification of trimethoxybenzoic acid moiety in reserpine molecule with Methelute where the respective methyl ester is GLC monitored. The method succeeded in separating reserpine from the accompanying components in the pharmaceutical preparations and gave linear response curves. The method was successfully applied to the micro-determination of reserpine in various pharmaceutical preparations before and after the addition of standard reserpine