use of enzyme-linked immunosorbent assay [ELISA] for quantitative detection of potato virus Y in potato and other test plants.

ABSTRACT

In this study, ELISA was used to detect potato virus Y [PVY] in crude extracts of virus preparations from artificially infected leaves and tuber sprouts of different potato cultivars as well as in extracts and intact leaf discs of some hosts such as potato hybrid A6, tobacco, wild tobacco, tomato, wild tomato, pepper, Physalis floridana, Datura metel, D. Stramonium, Hyoscyamus muticus, Chenopodium amaranticolor, C. Quinoa, egg plant, Solanum nigrum, Gomphrena globosa and in some other herbaceous test plants. ELISA was a rapid and sensitive method enabling PVY to be detected three days after the inoculation of potato seedlings. The best stage of plant development for PVY detection in potato sprouts was 55 days after planting. It was possible to detect PVY by ELISA in intact leaf discs without the homogenization of Enab El-Deeb, potato, pepper, tomato, Datura metel, wild tobacco plants and gave a positive reaction with extracts containing PVY, but not with the other five plant viruses